盐穴地下储气库密封性的测试与评价

对盐穴储气库系统密封性的测试与评价方法进行了详细介绍。测试方法选取原则、测试相关计算方法以及测试标准为储气库密封性测试的工程设计提供了详实的理论基础。     

An engineering method for complex structural optimization involving both size and topology design variables

This work presents an engineering method for optimizing structures made of bars, beams, plates, or a combination of those components. Corresponding problems involve both continuous (size) and discrete (topology) variables. Using a branched multipoint approximate function, which involves such mixed variables, a series of sequential approximate problems are constructed to make the primal problem explicit. To solve the approximate problems, genetic algorithm (GA) is utilized to optimize discrete variables, and when calculating individual fitness values in GA, a second-level approximate problem only involving retained continuous variables is built to optimize continuous variables. The solution to the second-level approximate problem can be easily obtained with dual methods. Structural analyses are only needed before improving the branched approximate functions in the iteration cycles. The method aims at optimal design of discrete structures consisting of bars, beams, plates, or other components. Numerical examples are given to illustrate its effectiveness, including frame topology optimization, layout optimization of stiffeners modeled with beams or shells, concurrent layout optimization of beam and shell components, and an application in a microsatellite structure. Optimization results show that the number of structural analyses is dramatically decreased when compared with pure GA while even comparable to pure sizing optimization.     

3D printing hybrid organometallic polymer‐based biomaterials via laser two‐photon polymerization

Materials with microscale structures are gaining increasing interest due to their range of technical and medical applications. Additive manufacturing approaches to such objects via laser two‐photon polymerization, also known as multiphoton fabrication, enable the creation of new materials with diverse and tunable properties. Here, we investigate the properties of 3D structures composed of organometallic polymers incorporating aluminium, titanium, vanadium and zirconium. The organometallic polymer‐based materials were analysed using a variety of techniques including SEM, energy‐dispersive X‐ray spectroscopy, X‐ray photoelectron spectroscopy analysis and contact angle measurements and their biocompatibility was tested in vitro. Cell viability and mode of death were determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay and acridine orange/ethidium bromide staining. Polymers incorporating Al, Ti and Zr supported cell adhesion and proliferation, and showed low toxicity in vitro, whereas the organometallic polymer incorporating V was shown to be cytotoxic. Inductively coupled plasma optical emission spectrometry suggested that leaching of the V from the organometallic polymer is the likely cause of this. The preparation of the organometallic polymers is straightforward and both simple 2D and complex 3D structures can be fabricated with ease. Resolution tests of the newly developed organometallic polymer incorporating Al show that suspended lines with widths down to 200 nm can be fabricated. We believe that the materials described in this work show promising properties for the development of objects with sub‐micron features for biomedical applications (e.g. biosensors, drug delivery devices, tissue scaffolds etc.). © 2019 The Authors. Polymer International published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

爆炸防控技术在爆破工程警务规划和技术评估中应用

通过介绍爆炸防控技术概念的内涵和外延,说明了爆破工程警务规划设计和技术评估的目的,就是有效防止或遏制涉爆事故,维护社会公共安全。在对一项复杂环境下的基础开挖爆破工程实施警务规划设计和技术评估时,警务技术民警对照爆破作业设计施工单位技术方案中的参数,现场核实需要保护的建筑和重要设施,准确测量保护目标与爆区的实际距离,分析是否存在影响爆破工程实施的不稳定因素,确认上述参数符合安全要求后做出准予爆破许可的建议。爆破工程警务规划设计和技术评估的要点就是要通过现场核查,核实爆破有害效应是否可控,能否确保爆破施工时周边保护目标的安全。     

Catalytic biohydrogen production from organic waste materials: A literature review and bibliometric analysis

Global population growth and accelerated urbanisation have resulted in massive amounts of fossil fuel use and waste production. Because of its high energy content, pure nature, and fuel quality, hydrogen fuel is a viable option to fossil fuels. Biohydrogen from agricultural waste, in particular, piques concern because it generates hydrogen while still disposing of waste. This review conducted a bibliometric analysis of biohydrogen production from organic waste to trace the research trends and hotspots based on the literature in the Web of Science (WOS) database from 1970 to 2020. The present review article also focuses on highlighting various processes for converting organic waste into hydrogen, raw materials for biohydrogen production, and catalysts that could distil the latest perceptions that could shed light on a route advancing for successful catalyst design. It also seems that some intentions have been paid on studying waste materials such as pure polysaccharides, disaccharides, and monosaccharides. Among all the catalysts used, non-noble and low-cost active metals over reduced graphene oxide (rGO) support can significantly affect the activity of fermentative hydrogen production from organic waste materials. However, researches focusing on developing anaerobic membrane bioreactors for these technologies are still needed.     

Ontology-based approach for the provision of simulation knowledge acquired by Data and Text Mining processes

Numerical simulation techniques such as Finite Element Analyses are essential in today's engineering design practices. However, comprehensive knowledge is required for the setup of reliable simulations to verify strength and further product properties. Due to limited capacities, design-accompanying simulations are performed too rarely by experienced simulation engineers. Therefore, product models are not sufficiently verified or the simulations lead to wrong design decisions, if they are applied by less experienced users. This results in belated redesigns of already detailed product models and to highly cost- and time-intensive iterations in product development.Thus, in order to support less experienced simulation users in setting up reliable Finite Element Analyses, a novel ontology-based approach is presented. The knowledge management tools developed on the basis of this approach allow an automated acquisition and target-oriented provision of necessary simulation knowledge. This knowledge is acquired from existing simulation models and text-based documentations from previous product developments by Text and Data Mining. By offering support to less experienced simulation users, the presented approach may finally lead to a more efficient and extensive application of reliable FEA in product development.     

Allele-Specific Inhibition of Histone Demethylases

Histone demethylases play a critical role in mammalian gene expression by removing methyl groups from lysine residues in degree- and site-specific manner. To specifically interrogate members and isoforms of this class of enzymes, we have developed demethylase variants with an expanded active site. The mutant enzymes are capable of performing lysine demethylation with wild-type proficiency, but are sensitive to inhibition by cofactor-competitive molecules embellished with a complementary steric “bump”. The selected inhibitors show more than 20-fold selectivity over the wild-type demethylase, thus overcoming issues typical to pharmacological and genetic approaches. The mutant–inhibitor pairs are shown to act on a physiologically relevant full-length substrate. By engineering a conserved amino acid to achieve member-specific perturbation, this study provides a general approach for studying histone demethylases in diverse cellular processes.     

Professional design of chemical plants based on problem-based learning on a pilot plant

This work describes a novel teaching methodology and its application in a subject framed in the last course of the master’s degree in Chemical Engineering at the University of Seville. The main aim consists of promoting a way to facilitate professional practice in this field to the students. Authors also aim at guiding the transition to the new learning requirements demanded by the European Higher Education Area (EHEA).The novel methodology is based on the development of a detailed design of a pilot plant that can be further evaluated by the students themselves. Students are also allowed to run experiments at the facility and carry out several standard tests to verify the quality of the results of their works. The novelty of this proposal consists of incorporating a real installation available in our department to drive new learning methodologies applied in Chemical Engineering curricula. The methodology is distributed across several tasks: training (self-learning), project execution and supervision, comparison with a real installation and pilot plant experiences. The application of the proposed teaching methodology provided quality didactic material production, high satisfaction of the students with the new learning approach and the fact that they gained practical experience at industrial scale.     

Pulsed Electromagnetic Field Stimulation in Osteogenesis and Chondrogenesis: Signaling Pathways and Therapeutic Implications

Mesenchymal stem cells (MSCs) are the main cell players in tissue repair and thanks to their self-renewal and multi-lineage differentiation capabilities, they gained significant attention as cell source for tissue engineering (TE) approaches aimed at restoring bone and cartilage defects. Despite significant progress, their therapeutic application remains debated: the TE construct often fails to completely restore the biomechanical properties of the native tissue, leading to poor clinical outcomes in the long term. Pulsed electromagnetic fields (PEMFs) are currently used as a safe and non-invasive treatment to enhance bone healing and to provide joint protection. PEMFs enhance both osteogenic and chondrogenic differentiation of MSCs. Here, we provide extensive review of the signaling pathways modulated by PEMFs during MSCs osteogenic and chondrogenic differentiation. Particular attention has been given to the PEMF-mediated activation of the adenosine signaling and their regulation of the inflammatory response as key player in TE approaches. Overall, the application of PEMFs in tissue repair is foreseen: (1) in vitro: to improve the functional and mechanical properties of the engineered construct; (2) in vivo: (i) to favor graft integration, (ii) to control the local inflammatory response, and (iii) to foster tissue repair from both implanted and resident MSCs cells.     

新工科背景下电子通信专业“人工智能技术基础”课程改革实践

人工智能技术已经成为新一轮科技革命的重要驱动力量，这要求电子、通信等非计算机专业的人才培养引入针对性强的人工智能课程教学。本文针对新工科背景下电子、通信专业“人工智能技术基础”课程存在的问题进行课程改革，从授课和实验两个环节入手，分别从教学内容、教学方法和考核办法三个方面提出了具体的改革举措，并在“人工智能技术基础”课程中进行了实践，激发了学生的学习热情，拓展了学生的专业视野，提高了学生的实践创新能力，取得了良好的教学效果。为了进一步培养电子、通信学科交叉型AI人才，该课程选拔优秀学生开展丰富的人工智能课外实践活动，进一步提升学生的实践创新能力。